Content of phenolic compounds in leaves of trees growing on devastated lands at iron waste rock dump

Knowledge about the content of phenolic compounds in tree leaves growing on devastated lands may be important for foresting post-mining landscapes and improving environmental quality industrial areas. The object this study was standpoint an ecosystem approach to determine concentrations trees that grow naturally at iron waste rock dump. Petrovsky dump areas Kryvyi Rih Iron Ore Mining & Metallurgical District (central part Ukraine) were chosen present research. During 2017–2021, 5 research plots dump’s area 1 plot background (natural ecosystems Hurivskyi forest, Kirovohradska oblast) established. investigated three woody plants species: silver birch (Betula pendula Roth.), ashleaf maple (Acer negundo L.) black locust (Robinia pseudoacacia L.). extracts prepared by extraction 80% isopropanol. phenols concentration determined using Folin-Ciocalteu reagent. absorbance measured spectrophotometer wavelength 725 nm. calibration curve constructed standard solutions gallic acid. expressed as acid equivalents milligrams per gram dry weight plant material (mg GAE * g-1 dw). results showed values vary within minimal limits: from 26,90 27,49 mg dw birch, 17,53 18,11 10,98 11,65 locust. In all samples, generally natural limits. below levels. According analyses, species statistical significant differences than reference samples (P < 0,05). levels found greater 0,05) 15–65% 115–165%, respectively. lower (about 10–28%, P those recorded control. according resistance following decreasing order: Roth.) >